Journal: International Journal of Molecular Sciences
Article Title: Cervicovaginal Microbiome and HPV: A Standardized Approach to 16S/ITS NGS and Microbial Community Profiling for Viral Association
doi: 10.3390/ijms26168090
Figure Lengend Snippet: Taxonomic profiles of the ATCC vaginal microbiome standard and six cytology samples representing Community State Types (CST) I-V. ( A ) Six microbial species in the ATCC standard (each at 16.7%) were sequenced in replicate across V-regions and shown as stacked bars. Species-level specificity varied by region: only V1V2 detected L. gasseri , while V4V5 missed L. jensenii . V3V4 showed the most balanced representation across replicates. ( B ) Stacked bar plots show taxonomic profiles from six cytology samples representing CST I–V, each dominated, respectively, by L. crispatus , L. gasseri , L. iners , mixed anaerobic bacteria, and L. jensenii . Species-level detection varied by V-region: L. crispatus (CST I) was identified only by V1V2 and V7V9; L. gasseri (CST II) only by V1V2; L. iners (CST III) was consistently detected across all regions; and L. jensenii (CST IV-A and V) was absent in V4V5.
Article Snippet: The product includes an even mixture of genomic DNA from six bacteria (16.7% G. vaginalis (ATCC 14019D-5), 16.7% L. gasseri (ATCC 33323D-5), 16.7% M. hominis (ATCC 23114D-5), 16.7% P. bivia (ATCC 29303D-5), 16.7% S. agalactiae (ATCC BAA-611D-5), and 16.7% L. jensenii (ATCC 25258D-5)).
Techniques: Bacteria